Changes in the amino acid pool of cerebral hemispheres in rats with total cerebral ischemia

Year - Volume - Issue
Elizaveta I. Bon, Natalia Y. Maksimovich , Vitaly Y. Smirnov 1 , Evgeniy M. Doroshenko , Yuri E. Razvodovsky , Ekaterina V. Moroz , Elizaveta I. Vishnevskaya , Nikita V. Kokhan
Article type
Objective: assessment of changes in the pool of amino acids (AA) in rats with totalcerebral ischemia (TCI).
Materials and Methods. Experiments were performed on 16 male outbred white rats weighing 260±20 g. TCI was modeled by decapitation of animals. Brain tissue was sampled 1 hour after decapitation.
Results. In the parietal lobe (PL) and hippocampus (HC) of TCIgroup animals 1 hour aftertheir decapitation, we detected an increase in the content of tyrosine (by 43%, p=0.044, and 40%, p=0.044, respectively) and tryptophan (by 24%, p=0. 036, and 23%, p=0.046, respectively). Similar trend was observed for methionine that increased by 32% in PL (p=0.046) and by 27% in HC (p=0.046). Analogous increase in the content of L-arginine was noted in PL and HC (by 20%, p=0.037, and 33%, p=0.037, correspondingly). Isoleucine content increased by 12% in PL (p=0.054), whilevaline content decreased by 15% in HC (p=0.053). The ratio of the combined total content of branched-chain amino acids (BCAA) to the combined content of aromatic AA in TCI significantly declined from 1.4 to 1.0 in PL (p=0.053) and from 1.6 to 1.0 in GC (p=0.053). We observed an increase in methionine content by 33% (p=0.046) in PL and an increase in tryptophan content by 24% (p=0.046) in HC.
Conclusion. One-hour TCIcaused the following changes in the AA pool: an increase in the content of aromatic AA (tyrosine and tryptophan) and methionine; an increase in the content of L-arginine; and also, an increase in the concentration of the inhibitory neurotransmitter glycine.
Cite as
Bon EI, Maksimovich NYe, Smirnov VYu, Doroshenko EM, Razvodovsky YuE, Moroz EV, Vishnevskaya EI, Kokhan NV.
Changes in the amino acid pool of cerebral hemispheres in rats with total cerebral ischemia. Saratov Medical Journal 2023; 4 (4): e0401.
  1. Berlucchi G, Vallar G. The history of the neurophysiology and neurology of the parietal lobe. Handb Clin Neurol. 2018; 151:3-30.

  2. Shimizu H, Graham SH, Chang LH, et al. Relationship between extracellular neurotransmitter amino acids and energy metabolism during cerebral ischemia in rats monitored by microdialysis and in vivo magnetic resonance spectroscopy. Brain Research 1993; 605(1):33-42.

  3. Li JS, Qiu HM, Jiang QS et al. Changes in the levels of 18 amino acids in the cortex and plasma of cerebral ischemia in C57BL/6 mice. Biomed Chromatogr. 2021; 35(6): e5084.

  4. Bon E, Maksimovich N, Karnyushko S, et al. Disorders of energy metabolism in neurons of the cerebral cortex during cerebral ischemia. Biomedical Journal of Scientific & Technical Research 2021; 40 (1): 31932-31937. 

  5. Bon EI, Maksimovich NE, Dremza IK, et al. Characterizing a disorder of prooxidant-oxidant balance in rats with cerebral ischemia. Ulyanovsk Journal on Medical Technology 2022; 3: 97-106. [In Russ.].

  6. Maksimovich NE, Bon EI, Zimatkin SM, et al. The Cerebral Cortex of Rats and its Response to Ischemia. Grodno: Grodno State Medical University Publishers 2020; 240. [In Russ.].

  7. Razvodovsky YuE, Razvodovsky YuE, Doroshenko EM, et al. The content of amino acids and their derivatives in the cerebral cortex of rats with incomplete cerebral ischemia. Bulletin of Smolensk State Medical Academy 2019; 18 (1): 5-9. [In Russ.].

  8. Sun W, Chen Y, Zhang Y, et al. Modified four-vessel occlusion model of global cerebral ischemia in rats. Journal of Neuroscience Methods 2021; 352: 109090.

  9. Weltkamp R, Rajapakse N, Robins G, et al. Transient focal ischemia increases endothelial nitric oxide synthase in cerebral blood vessels. Stroke. 2002; 33(11): 2704-2710.

  10. Fernstrom DD. Branched chain amino acids and brain function. J Nutr. 2005; 135: 1539S-46S.

  11. Gibson GE, Peterson S, Sanson J. Decreased amino acid and acetylcholine metabolism during hypoxia. J Neurochem. 1981; 37(1): 192-201.

  12. Erecińska M, Nelson D, Wilson DF, Silver IA. Neurotransmitter amino acids in the CNS. I. Regional changes in amino acid levels in rat brain during ischemia and reperfusion. Brain Res. 1984; 304(1):9-22.

  13. Razvodovsky YuE, Smirnov VY, Doroshenko YM, et al. Effect of blockade of synthesis of nitric oxide by L-NAME on the level of free amino acids and biogenic amines in the brain cortex of rats with subtotal cerebral ischemia. Proceedings of the National Academy of Sciences of Belarus, Medical series 2019; 16 (3): 291-297.

About the Authors

Elizaveta I. Bon –PhD, Associate Professor, Department of Pathophysiology, 0000-0001-7189-0838.

Natalia Y. Maksimovich – DSc, Professor, Chair of the Department of Pathophysiology, ; 

Vitaly Y. Smirnov –PhD, Senior Researcher of the research laboratory, ; 

Evgeniy M. Doroshenko – PhD, Lead Researcher of the research laboratory, ; 

Yuri E. Razvodovsky – PhD, Head of the Division,; 

Ekaterina V. Moroz –Student, Grodno State Medical University,; 

Elizaveta I. Vishnevskaya – Student, Grodno State Medical University;

Nikita V. Kokhan –Student, Grodno State Medical University, ,  0009-0009-8000-803X.



Received 6  May, 2023, Accepted 24 November 2023